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KMID : 1094020150320020158
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Journal of Veterinary Clinics
2015 Volume.32 No. 2 p.158 ~ p.161
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Comparison of Harboring the Resistance Gene and Disc Diffusion Susceptibility Test Result in Staphylococcus pseudintermedius from the Bacterial Dermatitis
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Jang Hye-Jin
Son Hyoung-Won
Kang Hyo-Min
Na Ki-Jeong
Han Jae-Ik
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Abstract
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Bacterial dermatitis is common disease that is necessary to treat with antibiotics. In recent, antibiotic-resistantbacteria is being increased in worldwide. The purpose of the present study was to evaluate the prevalence of resistantgenes in Staphylococcus (S.) pseudintermedius isolated from dogs, and to compare the resistant gene profile with theresult of antibiotic disc diffusion test. A total of seven S. pseudintermedius was included in the study. Bacterialidentification was performed by 16S ribosomal RNA gene sequence analysis. S. pseudintermedius isolates had morethan one antibiotic resistant gene (mecA, blaZ and aac(6')/aph(2"). While all isolates were PCR positive to blaZ gene,only two isolates were resistant to amoxicillin/clavulanate. Among five isolates harboring gentamicin resistance, oneisolate was negative to aac(6')/aph(2")-targeted PCR. Taken together, the results suggest that resistant gene-targetedPCR and disc diffusion test are complementary to detect antibiotic resistance.
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KEYWORD
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antibiotic susceptibility, bacterial dermatitis, resistance gene, dog
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